Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1992 Mar;11(2):105-8.
doi: 10.1007/BF00235264.

Transient gene expression in strawberry (Fragaria x ananassa Duch.) protoplasts and the recovery of transgenic plants

M Nyman  1 , A Wallin
Affiliations

Transient gene expression in strawberry (Fragaria x ananassa Duch.) protoplasts and the recovery of transgenic plants

M Nyman et al. Plant Cell Rep. 1992 Mar.

Abstract

A transient ß-glucuronidase (GUS)-assay was performed to evaluate electroporation parameters and optimize DNA delivery conditions into strawberry protoplasts. Optimal GUS-activity was obtained when protoplasts were subjected to 400 V/cm for 20 ms. GUS-activity could be further increased by the addition of carrier DNA to the electroporation mixture. Callus selected on 10 μg/ml hygromycin produced shoots which exhibited GUS-activity. The transformed nature of the shoots obtained after selection was confirmed by DNA-analysis.

PubMed Disclaimer

References

    1. Plant Cell Rep. 1988 Dec;7(7):481-4 - PubMed
    1. Plant Cell Rep. 1987 Jul;6(4):265-70 - PubMed
    1. Science. 1988 Apr 8;240(4849):204-7 - PubMed
    1. Proc Natl Acad Sci U S A. 1985 Sep;82(17):5824-8 - PubMed
    1. EMBO J. 1987 Dec 20;6(13):3901-7 - PubMed

LinkOut - more resources