Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1991 Jan;9(9):475-8.
doi: 10.1007/BF00232099.

Rescuing abortive inter-EBN potato hybrids through double pollination and embryo culture

Affiliations

Rescuing abortive inter-EBN potato hybrids through double pollination and embryo culture

C Singsit et al. Plant Cell Rep. 1991 Jan.

Abstract

Regeneration of inter-EBN hybrids among potato species was achieved using embryo rescue techniques. Tetraploid hybrids between 4x(2EBN) Solanum stoloniferum x 4x(4EBN) S. tuberosum Gp. Andigena as well as diploid hybrids between 2x(1EBN) S. chancayense x 2x(2EBN) S. chacoense were obtained by culturing immature hybrid embryos in nutrient medium. Identification of appropriate embryo developmental stages was critical in developing a suitable protocol for rescuing viable hybrid embryos. The use of IVP clones as the second pollinator in 4x(2EBN) x 4x(4EBN) crosses reduced premature fruit drop and helped to identify triploid hybrids. Morphological and cytological examination confirmed true hybridity for a few of the regenerated plants. Male sterility and meiotic abnormalities were characteristic of the hybrids. Several S. stoloniferum-Andigena hybrids were successfully backcrossed to Gp. Andigena.

PubMed Disclaimer

References

    1. Theor Appl Genet. 1988 Jul;76(1):129-35 - PubMed
    1. Theor Appl Genet. 1988 Aug;76(2):260-6 - PubMed
    1. Plant Cell Rep. 1984 Aug;3(4):165-7 - PubMed
    1. Theor Appl Genet. 1968 Apr;38(4):153-67 - PubMed