The immunogold staining technique for the measurement of lymphocyte subpopulations in bronchoalveolar lavage fluid

Abstract

The immunogold staining technique was evaluated for use in the identification of lymphocyte subsets in bronchoalveolar lavage fluid. The results obtained compared favorably to the identification of lymphocytes by the E-rosette method, the acid alpha-naphthyl-acetate esterase (ANAE) stain and immunofluorescence microscopy. The main advantages of the immunogold staining method include improved cellular detail and the attainment of permanent preparations, allowing for reassessment, intralaboratory comparison and the performance of further histochemical techniques if desired.