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. 1990 Aug;80(2):241-5.
doi: 10.1007/BF00224393.

Isolation of EMS-induced mutants in Arabidopsis altered in seed fatty acid composition

D W James Jr  1 , H K Dooner
Affiliations

Isolation of EMS-induced mutants in Arabidopsis altered in seed fatty acid composition

D W James Jr et al. Theor Appl Genet. 1990 Aug.

Abstract

Mutants of Arabidopsis thaliana were identified by screening pedigreed M3 seed collections from EMS-treated plants for changes in fatty acid (FA) composition. The FA phenotypes of the most dramatic mutants are as follows: G30 and 1E5 (allelic) lack linolenic acid (18∶3) and are elevated in linoleic acid (18∶2); 4A5 is deficient in 18∶2 and 18∶3 and fourfold increased in oleic acid (18∶1); 9A1 lacks all FAs > C18 and is twofold increased in 18∶1; 1A9 is twofold increased in palmitic acid (16∶0) and decreased by one-half in 18∶1; 2A11 is two-to threefold increased in stearic acid (18∶0) and decreased by one-half in 18∶1. Based on segregation of F2 selfed plants derived from crosses to wild type, all of these phenotypes are the result of single gene mutations.

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References

    1. Proc Natl Acad Sci U S A. 1988 Jun;85(12):4143-7 - PubMed
    1. Theor Appl Genet. 1990 Aug;80(2):234-40 - PubMed
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