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. 1994 Jul;5(7):623-31.
doi: 10.1016/1044-0305(94)85003-8.

Gas-phase hydrogen/deuterium exchange as a molecular probe for the interaction of methanol and protonated peptides

E Gard  1 , M K Green, J Bregar, C B Lebrilla
Affiliations

Gas-phase hydrogen/deuterium exchange as a molecular probe for the interaction of methanol and protonated peptides

E Gard et al. J Am Soc Mass Spectrom. 1994 Jul.

Abstract

The gas-phase hydrogen/deuterium (HID) exchange kinetics of several protonated amino acids and dipeptides under a background pressure of CH3OD were determined in an external source Fourier transform mass spectrometer. H/D exchange reactions occur even when the gas-phase basicity of the compound is significantly larger (> 20 kcal/mol) than methanol. In addition; greater deuterium incorporation is observed for compounds that have multiple sites of similar basicities. A mechanism is proposed that involves a structurally specific intermediate with extensive interaction between the protonated compound and methanol.

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References

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