Evidence from capping experiments for independence of the RT7 alloantigen and the leucocyte common antigen in the rat

Abstract

Experiments reported here demonstrate that the RT7 alloantigen and the L-C antigen are separate and distinct structures on the surface of rat lymphocytes. The distribution of the antigens in different rat strains, including the mutant WF/fz, clearly establish the RT7 antigenic system as a polymorphic diallelic system, whereas the recognized L-C antigenic determinant is monomorphic and present on the lymphocytes of all rat strains tested. These data were obtained using monoclonal antibodies to the antigens in indirect immunofluorescence experiments. The two antigens were shown to redistribute (cap) independently of one another on the surface of rat thymocytes. Cells that had been exposed to anti-L-C antibody and FITC-conjugated anti-Ig followed by anti-RT7.1 antibody and RITC-conjugated anti-Ig demonstrated FITC caps and RITC rings.