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. 2013 Dec;35(10):993-1001.
doi: 10.1179/016164113X13756993777580.

SELDI-TOF analysis of glioblastoma cyst fluid is an approach for assessing cellular protein expression

Martin Hoelscher  1 , Nina Richter, Christian Melle, Ferdinand von Eggeling, Anne Schaenzer, Ulf Nestler
Affiliations
Free PMC article

SELDI-TOF analysis of glioblastoma cyst fluid is an approach for assessing cellular protein expression

Martin Hoelscher et al. Neurol Res. 2013 Dec.
Free PMC article

Abstract

Objectives: In about 10% of glioblastoma patients, preoperative MRI discloses the presence of tumor cysts. Whereas the impact of cystic appearance on prognosis has been discussed extensively, only little is known about the tumor cyst fluid. In this study, we tested the feasibility of the surface enhanced laser desorption ionization time of flight (SELDI-TOF) technique to detect cyst fluid proteins.

Methods: Cyst fluid was collected from 21 glioblastoma patients for SELDI-TOF analysis and compared to control cerebrospinal fluids from 15 patients with spinal stenosis. Resulting protein peaks with significant differences between groups were further described, using the molecular weight in an internet search of protein databases and publications. Two potential cyst fluid proteins, basigin and ferritin light chain, were selected for immunohistological detection in the histologic slides of the patients, metallothionein (MT) served as negative control.

Results: As supposed from the results of the SELDI-TOF analysis, basigin and ferritin were detected immunohistochemically in the cyst wall, whereas MT was more equally distributed between the cyst wall and the surrounding tumor tissue. Median survival time of the patients was 20 months (range 2 to 102 months) and correlated with age, but not with expression of the three proteins.

Discussion: The SELDI-TOF approach reveals a number of proteins, potentially present in glioblastoma cyst fluid. Identification of these proteins in tumor cells may help understand the pathogenetic pathways and the prognostic value of cystic changes.

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Figures

Figure 1
Figure 1
SELDI-TOF chromatographic results for four tumor cyst fluids #14 to #17 and one cerebrospinal fluid control #L6. The molecular weight of the peaks in daltons is given on the horizontal axis and the height of the peaks can be compared in micro-amperes on the vertical axis, as shown here for albumin and double loaded albumin.
Figure 2
Figure 2
A–C) Basigin (CD147) staining of the cyst wall (original magnification 10×, 20×, and 40×, respectively). (D–F) Ferritin staining. (G–I) MT staining. Immunohistologic staining of two different glioblastoma cyst specimens (case 1: A, D, G and case 2: B, C, E, F, H, I). Basigin and ferritin are strongly expressed in the cyst walls, whereas metallothionein (MT) staining is less prominent and more equally distributed between the cyst wall and the main tumor tissue.
See this image and copyright information in PMC

References

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