Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1990 Jan;79(1):49-55.
doi: 10.1007/BF00223786.

High transmission of paternal plastid DNA in alfalfa plants demonstrated by restriction fragment polymorphic analysis

S A Masoud  1 , L B Johnson, E L Sorensen
Affiliations

High transmission of paternal plastid DNA in alfalfa plants demonstrated by restriction fragment polymorphic analysis

S A Masoud et al. Theor Appl Genet. 1990 Jan.

Abstract

A high frequency of paternal plastid transmission occurred in progeny from crosses among normal green alfalfa plants. Plastid transmission was analyzed by hybridization of radiolabeled alfalfa plastid DNA (cpDNA) probes to Southern blots of restriction digests of the progeny DNA. Each probe revealed a specific polymorphism differentiating the parental plastid genomes. Of 212 progeny, 34 were heteroplastidic, with their cpDNAs ranging from predominantly paternal to predominantly maternal. Regrowth of shoots from heteroplasmic plants following removal of top growth revealed the persistence of mixed plastids in a given plant. However, different shoots within a green heteroplasmic plant exhibited paternal, maternal, or mixed cpDNAs. Evidence of maternal nuclear genomic influence on the frequency of paternal plastid transmission was observed in some reciprocal crosses. A few tetraploid F1 progeny were obtained from tetraploid (2n=4x=32) Medicago sativa ssp. sativa x diploid (2n=2x=16) M. sativa ssp. falcata crosses, and resulted from unreduced gametes. Here more than the maternal genome alone apparently functioned in controlling plastid transmission. Considering all crosses, only 5 of 212 progeny cpDNAs lacked evidence of a definitive paternal plastid fragment.

PubMed Disclaimer

References

    1. Proc Natl Acad Sci U S A. 1987 Apr;84(7):2097-100 - PubMed
    1. Stain Technol. 1963 Jan;38:9-13 - PubMed
    1. Plant Mol Biol. 1989 Jan;12(1):87-93 - PubMed
    1. Theor Appl Genet. 1981 Jan;60(1):37-41 - PubMed
    1. Plant Physiol. 1982 Dec;70(6):1710-4 - PubMed