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. 1990 Sep;9(5):253-6.
doi: 10.1007/BF00232295.

Fertile Indica rice plants regenerated from protoplasts isolated from microspore derived cell suspensions

S K Datta  1 , K Datta, I Potrykus
Affiliations

Fertile Indica rice plants regenerated from protoplasts isolated from microspore derived cell suspensions

S K Datta et al. Plant Cell Rep. 1990 Sep.

Abstract

Rice plants (Oryza sativa L., Chinsurah Boro II var. Indica) were regenerated from protoplasts isolated from microspore derived cell suspensions. A simple procedure for the establishment of such cell suspension cultures from embryogenic microcallus derived from cultured isolated microspores of Indica-type rice is described. Regenerating protoplasts could readily be isolated from 5-12 months old cell suspensions showing visible colony formation in the range of 180-1050 colonies/10(6) protoplasts after about one month in culture. More than 100 independent green plantlets were regenerated via secondary embryogenesis from ca 20×10(6) protoplasts. Out of 32 plants grown to maturity under greenhouse conditions 24 were fertile.

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