Genome-wide identification and analysis of FK506-binding protein family gene family in strawberry (Fragaria × ananassa)

Abstract

The FK506 binding proteins (FKBPs) are abundant and ubiquitous proteins belonging to the large peptidyl-prolylcis-trans isomerase superfamily. FKBPs are known to be involved in many biological processes including hormone signaling, plant growth, and stress responses through a chaperone or an isomerization of proline residues during protein folding. The availability of complete strawberry genome sequences allowed the identification of 23 FKBP genes by HMMER and blast analysis. Chromosome scaffold locations of these FKBP genes in the strawberry genome were determined and the protein domain and motif organization of FaFKBPs analyzed. The phylogenetic relationships between strawberry FKBPs were also assessed. The expression profiles of FaFKBPs genes results revealed that most FaFKBPs were expressed in all tissues, while a few FaFKBPs were specifically expressed in some of the tissues. These data not only contribute to some better understanding of the complex regulation of the strawberry FKBP gene family, but also provide valuable information for further research in strawberry functional genomics.

Keywords: Bioinformatics analysis; CDS; CaM-BDs; ER; FK506-binding domain; FK506-binding proteins; FKBPs; FKBd; HSP90; MD; PPIase; RT-PCR; SD; Sequence express analysis; Strawberry; TOR; TPR; calmodulin-binding domains; coding sequence; endoplasmic reticulum; heat streak protein 90; multidomain; peptidyl-prolylcis-trans isomerase; semi-quantitative reverse transcription PCR; single-domain; target of rapamycin; tetratricopeptide repeat.