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. 1986 Sep;168(3):386-9.
doi: 10.1007/BF00392365.

Patatin, a major soluble protein of the potato (Solanum tuberosum L.) tuber is synthesized as a larger precursor

B Krischner  1 , H Hahn
Affiliations

Patatin, a major soluble protein of the potato (Solanum tuberosum L.) tuber is synthesized as a larger precursor

B Krischner et al. Planta. 1986 Sep.

Abstract

Antibodies were raised against the highly purified glycoprotein patatin. They were used to characterize the product synthesized in a wheatgerm cell-free translation system, programmed with polyadenylated RNA from potato tubers. Sodium dodecylsulfate-polyacrylamide gel electrophoresis revealed that the immunoprecipitated protein had a molecular mass of 43 kDa compared to 40 for the authentic patatin. It is assumed that patatin is synthesized in vivo as a larger precursor which is processed to the mature protein by cleavage of a signal peptide. Our results are in agreement with sequence-analysis data of patatin complementary DNA which indicate a signal peptide of about 23 amino acids (Mignery et al., 1984; Nucleic Acids Res. 12, 7987-8000).

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References

    1. Methods Enzymol. 1983;96:111-20 - PubMed
    1. Nucleic Acids Res. 1984 Nov 12;12(21):7987-8000 - PubMed
    1. Z Naturforsch C Biosci. 1983 Nov-Dec;38(11-12):1069-71 - PubMed
    1. Methods Enzymol. 1983;101:635-44 - PubMed
    1. Anal Biochem. 1978 Aug 15;89(1):264-73 - PubMed