Incidence of endogenous viral genes in two strains of white leghorn chickens selected for egg production and susceptibility or resistance to Marek's disease

Abstract

Endogenous viral (ev) genes related to the avian leukosis virus were classified in two differentially selected strains of Leghorns in order to investigate whether such genes affect production traits. Strain K had been selected for resistance to Marek's disease (MD) and for high egg production and egg weight, whereas strain S had been selected only for MD susceptibility. Except that founders of strain K included a few commercial birds, both strains were derived from a common genetic base. DNA restriction fragment length analyses of 110 strain K and 94 strain S birds revealed the presence of 8 different ev-genes, 6 of which were identical to previously identified loci. This result was confirmed by assays for group specific antigen (gs-antigen), the product of the gag region of the ev-genes. The levels of gs-antigen in the birds closely followed what had been predicted from data obtained from previously described ev-genes. Both strains had a similar average number of ev-genes per bird (3.5 and 3.2 for strains S and K, respectively). However, strain K carried only five different ev-genes while strain S carried seven. Four of these loci were present in both strains. Among the ev-genes absent or occurring less frequently in strain K were those that code either for infectious endogenous virus (ev-10 and possibly ev-19) or for the internal viral gag-proteins (ev-3). Only those ev-genes which are transcriptionally silent or which code for the viral envelope gene were present in increased frequencies in strain K. The results indicate that selection for egg traits and/or Marek's disease resistance reduces the frequency of ev-genes which produce endogenous virus or the viral gag-proteins.