Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1989 Jun;8(6):321-4.
doi: 10.1007/BF00716664.

Transformation of pea (Pisum sativum L.) byAgrobacterium tumefaciens

J Puonti-Kaerlas  1 , P Stabel, T Eriksson
Affiliations

Transformation of pea (Pisum sativum L.) byAgrobacterium tumefaciens

J Puonti-Kaerlas et al. Plant Cell Rep. 1989 Jun.

Abstract

Explants fromPisum sativum shoot cultures and epicotyls were transformed by cocultivation withAgrobacterium tumefaciens vectors carrying plant selectable markers and transformants could be selected on a medium containing kanamycin. Transformants could also be obtained at a low frequency by cocultivating small protoplast-derived colonies. The transformed nature of the calli obtained from selection was confirmed by opine assay and DNA analysis. In addition five cultivars of pea were tested for their response to seven differentAgrobacterium tumefaciens strains. The response pattern coincided largely between the different pea cultivars, being more dependent on the bacterial strain than the cultivar used.

PubMed Disclaimer

References

    1. Nucleic Acids Res. 1985 Oct 11;13(19):6981-98 - PubMed
    1. Biochim Biophys Acta. 1978 Dec 8;527(2):497-500 - PubMed
    1. DNA. 1986 Apr;5(2):101-13 - PubMed
    1. EMBO J. 1983;2(12):2143-50 - PubMed
    1. J Gen Virol. 1975 Jan;26(1):33-48 - PubMed

LinkOut - more resources