Regulated expression of repetitive sequences including the identifier sequence during myotube formation in culture

Abstract

We have isolated and characterized a cDNA of 1183 bp, pL6-411, from rat L6 muscle cells. This cDNA contains repetitive sequences - including two inverted copies of the previously described identifier sequence - as shown by sequence analysis. Repetitive sequences from pL6-411 characterize a family of RNAs which is specifically induced during L6 myotube formation. Another part of the pL6-411 sequence, existing at low-copy number per haploid rat genome, hybridized to two RNAs of 5 kb and 2 kb from L6 myoblasts as well as from L6 myotubes. A third pL6-411-related RNA of 150 bases was detected which hybridized with the repetitive sequence but did not hybridize with the low-copy number part of pL6-411. It appears that the 'identifier' sequence in this population of small RNAs is complementary to one of the 'identifier' copies in the pL6-411-related RNA. Finally, we identified on cDNA pL6-411 the recognition site for the TGGCA-binding protein and in both orientations a total of four putative promoters for RNA polymerase III.