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. 1994 Sep;4(3):255-8.
doi: 10.1007/BF01878460.

Triplet-state monitoring by fluorescence correlation spectroscopy

J Widengren  1 , R Rigler, U Mets
Affiliations

Triplet-state monitoring by fluorescence correlation spectroscopy

J Widengren et al. J Fluoresc. 1994 Sep.

Abstract

The effects of high excitation intensities in fluorescence correlation spectroscopy (FCS) in terms of saturation and triplet-state build-up have been studied for the case of Rh6G in aqueous solution. It was found that FCS provides a powerful means for the determination of intersystem crossing and triplet-state depopulation rates of fluorophores in solution.

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