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Comparative Study
. 1986 Jun;166(3):1106-12.
doi: 10.1128/jb.166.3.1106-1112.1986.

Incompatibility repressor in a RepA-like replicon of the IncFI plasmid ColV2-K94

P C Weber, S Palchaudhuri
Comparative Study

Incompatibility repressor in a RepA-like replicon of the IncFI plasmid ColV2-K94

P C Weber et al. J Bacteriol. 1986 Jun.

Abstract

The replication region Rep1 of the IncFI plasmid ColV2-K94 was cloned on self-replicating restriction fragments. Rep1 was structurally and functionally homologous to the RepA replicon of IncFII R plasmids. Despite this close relationship, these two replication systems were compatible with each other. The nucleotide sequence of the copA incompatibility-replication control gene of Rep1 was determined and compared with the copA sequence of RepA. Six base changes were found in a 24-base-pair span of the copA gene; these may result in the formation of a new, more stable, 49-base stem-loop structure in the potential CopA RNA repressor molecule. We postulate that these alterations weaken the interaction between RNA transcripts of the Rep1 and RepA replicons.

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References

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    1. Plasmid. 1985 May;13(3):215-8 - PubMed
    1. J Bacteriol. 1983 Apr;154(1):429-36 - PubMed
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