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. 1988 Oct;7(6):418-20.
doi: 10.1007/BF00269527.

Direct somatic embryogenesis from protoplasts of Citrus mitis Blanco

G E Sim  1 , C S Loh, C J Goh
Affiliations

Direct somatic embryogenesis from protoplasts of Citrus mitis Blanco

G E Sim et al. Plant Cell Rep. 1988 Oct.

Abstract

Protoplasts isolated from embryogenic suspension cultures of Citrus mitis were cultured in a medium without any plant growth substances. Somatic embryos developed directly from protoplasts without an obvious intervening callus phase. As many as 1,800 somatic embryos developed from 4 ml of protoplast suspension (density 2×10(6)/ml) cultured for 35 days. Upon transferring the embryoids to medium with 1 mgl(-1) GA3, they developed into plant-lets. Rooted plantlets were obtained in 3 months after protoplast isolation.

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