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. 1987 Jan;73(3):395-402.
doi: 10.1007/BF00262507.

Fertility of somatic hybrids from protoplast fusions of Solanum brevidens and S. tuberosum

M K Ehlenfeldt  1 , J P Helgeson
Affiliations

Fertility of somatic hybrids from protoplast fusions of Solanum brevidens and S. tuberosum

M K Ehlenfeldt et al. Theor Appl Genet. 1987 Jan.

Abstract

Two sets of somatic hybrids between Solanum brevidens (2x) and S. tuberosum (2x and 4x) were evaluated for male fertility, meiotic regularity and female fertility. The somatic hybrids were tetraploids from 2x + 2x fusions and hexaploids from 2x + 4x fusions. Pollen stainability ranged from 0 to 83% in tetraploids and from 0 to 23% in hexaploids. The tetraploids had more regular meiosis, lower levels of micropollen and fewer unassociated chromosomes than hexaploids. However, except for a low level of selfing, the pollen of both sets of hybrids was ineffective in pollinations. The tetraploids, as females, crossed poorly with 2x and 4x tester species and selfed only at low levels. The hexaploid fusion hybrids also crossed poorly with the 2x tester species and selfed only to a limited degree; however, they crossed well with 4x testers. Seed set in crosses with S. tuberosum Group Andigena, and S. tuberosum Group Tuberosum cultivars 'Kathadin' and 'Norland' averaged 16.7, 15.6 and 28.6 seeds per fruit, respectively. Progeny from these crosses had 5x or nearly 5x ploidy levels. The results indicate that reasonable levels of female fertility can be obtained in somatic fusion hybrids of S. brevidens and S. tuberosum.

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References

    1. Theor Appl Genet. 1984 May;68(1-2):155-61 - PubMed
    1. Plant Cell Rep. 1986 Jun;5(3):212-4 - PubMed
    1. Theor Appl Genet. 1980 Jan;57(1):5-9 - PubMed
    1. Science. 1982 Jul 30;217(4558):446-8 - PubMed
    1. Plant Cell Rep. 1984 Aug;3(4):165-7 - PubMed