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. 1985 Oct;166(2):151-5.
doi: 10.1007/BF00397341.

Separation of mitochondria from microbodies of Pisum sativum (L. cv. Alaska) cotyledons

N Burgess  1 , G W Beakes, D R Thomas
Affiliations

Separation of mitochondria from microbodies of Pisum sativum (L. cv. Alaska) cotyledons

N Burgess et al. Planta. 1985 Oct.

Abstract

A method is described for separating mitochondria from microbodies in cotyledon preparations of Pisum sativum L. cv. Alaska. Pure and intact mitochondria were obtained on a continuous: discontinuous sucrose density gradient as shown by marke-enzyme assay and electron microscopy. Manipulation of sucrose-gradient construction to widen the distance between organelles provided a quick method for the separation of the mitochondria from the microbodies. The shorter time of exposure of mitochondria to centrifugation and osmotic stress produces mitochondria free of contamination.

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