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. 1988 Mar;7(2):107-10.
doi: 10.1007/BF00270116.

Isolation and purification of generative cells from fresh pollen of Vicia faba L

C Zhou  1
Affiliations

Isolation and purification of generative cells from fresh pollen of Vicia faba L

C Zhou. Plant Cell Rep. 1988 Mar.

Abstract

A technique named two-step osmotic shock was developed for isolating generative cells (GCs): pollen grains were incubated in 20% sucrose solution and shocked by adding water. This caused the pollen grains to burst and release their contents including GCs. By subsequent filtration and centrifugation the isolated GCs were purified. The viability of GCs before and after purification was confirmed by fluorescein diacetate (FDA) test. This procedure offers a rapid and effective means to obtain living GCs in quantities.

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References

    1. Exp Cell Res. 1972 Jul;73(1):233-6 - PubMed
    1. Plant Physiol. 1986 May;81(1):317-9 - PubMed
    1. Plant Cell Rep. 1986 Aug;5(4):306-9 - PubMed
    1. Plant Physiol. 1987 Mar;83(3):464-6 - PubMed
    1. Exp Cell Res. 1971 Nov;69(1):29-32 - PubMed