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. 1988 Jun;7(4):225-8.
doi: 10.1007/BF00272529.

Transient gene expression in tobacco protoplasts: II. Comparison of the reporter gene systems for CAT, NPT II, and GUS

R Töpfer  1 , M Pröls, J Schell, H H Steinbiß
Affiliations

Transient gene expression in tobacco protoplasts: II. Comparison of the reporter gene systems for CAT, NPT II, and GUS

R Töpfer et al. Plant Cell Rep. 1988 Jun.

Abstract

The reporter genes for Chloramphenicolacetyltransferase (CAT), Neomycinphosphotransferase-(NPT)-II and β-Glucuronidase (GUS) were compared in transient gene expression experiments in tobacco mesophyll protoplasts. For this purpose, nearly identical chimeric genes controlled by the CaMV 35 S promoter were constructed. The detection level of each system was determined yielding the following order of relative sensitivity: CAT<NPT II<GUS.

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References

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