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. 1988 Jun;7(4):281-4.
doi: 10.1007/BF00272543.

Glyphosate tolerant flax plants from Agrobacterium mediated gene transfer

M C Jordan  1 , A McHughen
Affiliations

Glyphosate tolerant flax plants from Agrobacterium mediated gene transfer

M C Jordan et al. Plant Cell Rep. 1988 Jun.

Abstract

Agrobacterium tumefaciens carrying a disarmed Ti-plasmid vector containing a chimeric NPT-II gene and a glyphosate resistance plant-derived 5-enolpyruvylshikimate-3-phosphate synthase gene was used to transform flax hypocotyl tissues. Transformed shoots could be regenerated from the inoculated tissue and were proven to be transgenic by the combination of leaf callus assays, nopaline assays and progeny tests. Co-segregation was observed in the progeny for kanamycin and glyphosate resistance.

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References

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