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. 1985 Nov;71(1):1-4.
doi: 10.1007/BF00278245.

Somatic hybrid plants obtained by protoplast fusion between Citrus sinensis and Poncirus trifoliata

T Ohgawara  1 , S Kobayashi, E Ohgawara, H Uchimiya, S Ishii
Affiliations

Somatic hybrid plants obtained by protoplast fusion between Citrus sinensis and Poncirus trifoliata

T Ohgawara et al. Theor Appl Genet. 1985 Nov.

Abstract

Somatic hybrid plants of Rutaceae were obtained by protoplast fusion between Citrus sinensis Osb. ('Trovita' orange) and Poncirus trifoliata. Protoplasts isolated from embryogenic cells of C. sinensis and from leaves of P. trifoliata, and the culture of fusion products in the presence of high concentrations of sucrose were essential requirements for the selection of hybrids. Green globular embryoids derived from protoplasts resulted in the regeneration of trifoliate plants. Other morphological characters of these plants were intermediate between both parents. The chromosome number in one of the hybrid plants was 36, which was the sum of C. sinensis (2n=18) and P. trifoliata (2n=18). EcoRI restriction analysis of rDNA confirmed the presence of parental nuclear DNAs in the hybrid.

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References

    1. Theor Appl Genet. 1983 Jan;64(2):117-8 - PubMed
    1. Theor Appl Genet. 1982 Jun;62(2):171-6 - PubMed
    1. Theor Appl Genet. 1982 Mar;61(1):69-72 - PubMed

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