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. 1986 Apr;72(1):59-65.
doi: 10.1007/BF00261455.

Regeneration of somatic hybrid plants formed between Lycopersicon esculentum and Solatium rickii

M A O'Connell  1 , M R Hanson
Affiliations

Regeneration of somatic hybrid plants formed between Lycopersicon esculentum and Solatium rickii

M A O'Connell et al. Theor Appl Genet. 1986 Apr.

Abstract

A single somatic hybrid callus clone was identified following the fusion of Lycopersicon esculentum protoplasts and Solanum rickii suspension culture protoplasts. The hybrid nature of the callus and the plants regenerating from it was determined by assaying phosphoglucomutase-2 isozyme expression. The chloroplast genome present in four somatic hybrid plants was characterized by probing digests of total DNA with nick translated L. esculentum chloroplast DNA(cpDNA). All four somatic hybrid plants had inherited S. rickii cpDNA. Two clones of plant mitochondrial DNA (mtDNA), soybean 18S and 5S rDNA and maize cytochrome oxidase subunit II were used to characterize the mtDNA present in total DNA digests of four somatic hybrid plants. In both cases, the somatic hybrid plants had inherited most but not all of the S. rickii specific fragments, but none of the L. esculentum specific fragments.

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