doi: 10.1007/BF00269824.
An evaluation of Ficoll density gradient centrifugation as a method for eliminating microbial contamination and purifying plant protoplasts
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- PMID: 24248249
- DOI: 10.1007/BF00269824
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An evaluation of Ficoll density gradient centrifugation as a method for eliminating microbial contamination and purifying plant protoplasts
Plant Cell Rep.
1986 Aug.
Abstract
Ficoll density gradient centrifugation was used to generate sterile protoplasts from non-sterile Pteridium cultures. The method was found to drastically reduce levels of fungal and bacterial contamination introduced prior to centrifugation whilst concentrating and purifying protoplasts.
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