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. 1987 Jun;6(3):244-7.
doi: 10.1007/BF00268491.

Vacuolar yield and alteration with aging of suspension-cultured cells from pear fruit

S Yamaki  1 , R Romani
Affiliations

Vacuolar yield and alteration with aging of suspension-cultured cells from pear fruit

S Yamaki et al. Plant Cell Rep. 1987 Jun.

Abstract

Vacuoles were isolated from suspension-cultured pear fruit (Pyrus communis L. Passe Crassane) cells of various ages. Yield of vacuoles averaged 17% of protoplasts throughout all the experimental stages. The isolated vacuoles were progressively larger and more likely to stain with neutral red as cells advanced from division to growth and incipient senescence. A relationship between aging and vacuolar or membrane function was implicit in the size and staining characteristics of isolated vacuoles.

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