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. 1987 Jul;6(4):297-301.
doi: 10.1007/BF00272003.

Transformation of sugarcane protoplasts by direct uptake of a selectable chimaeric gene

W H Chen  1 , K M Gartland, M R Davey, R Sotak, J S Gartland, B J Mulligan, J B Power, E C Cocking
Affiliations

Transformation of sugarcane protoplasts by direct uptake of a selectable chimaeric gene

W H Chen et al. Plant Cell Rep. 1987 Jul.

Abstract

Sugarcane protoplasts were transformed to kanamycin resistance at a frequency of approximately 8 in 10(7) following PEG-induced uptake of Sma1 linearised pABD1 plasmid. DNA-treated protoplasts were cultured in agarose droplets, and protoplast-derived transformants selected on 80 μg ml(-1) kanamycin. Transformed tissues maintained on this level of antibiotic expressed APH(3')II activity, and contained DNA that hybridised to a probe with the APH(3')II gene.

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References

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