Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1987 Oct;6(5):396-9.
doi: 10.1007/BF00269571.

Genetic transformation of flax (Linum usitatissimum) by Agrobacterium tumefaciens: regeneration of transformed shoots via a callus phase

N Basiran  1 , P Armitage, R J Scott, J Draper
Affiliations

Genetic transformation of flax (Linum usitatissimum) by Agrobacterium tumefaciens: regeneration of transformed shoots via a callus phase

N Basiran et al. Plant Cell Rep. 1987 Oct.

Abstract

Genetic transformation of flax (Linum usitatissimum) has been achieved using an A. tumefaciens strain carrying a non-oncogenic Ti plasmid-derived vector containing a chimaeric npt-II gene and a wild type nopaline synthase gene. Fertile, transformed shoots were most easily obtained from Km(r) callus developing on hypocotyl sections. The totipotency of the Km(r) callus was dependent upon its origin. T-DNA was visualised by Southern blotting in all Km(r) tissues. Efficient expression of nopaline synthase and the chimaeric npt-II gene was found in transformed Km(r) callus and regenerated shoots.

PubMed Disclaimer

References

    1. Exp Cell Res. 1968 Apr;50(1):151-8 - PubMed
    1. Philos Trans R Soc Lond B Biol Sci. 1976 May 27;274(933):227-74 - PubMed
    1. Plant Cell Rep. 1983 Dec;2(6):314-7 - PubMed
    1. J Plant Physiol. 1984 Dec;117(2):109-17 - PubMed
    1. EMBO J. 1983;2(12):2143-50 - PubMed