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. 1987 Dec;6(6):476-9.
doi: 10.1007/BF00272787.

The isolation and culture of protoplasts from an embryogenic cell suspension culture of Picea glauca (moench) voss

F Bekkaoui  1 , P K Saxena, S M Attree, L C Fowke, D I Dunstan
Affiliations

The isolation and culture of protoplasts from an embryogenic cell suspension culture of Picea glauca (moench) voss

F Bekkaoui et al. Plant Cell Rep. 1987 Dec.

Abstract

Conditions were standardized for the isolation and culture of protoplasts from an embryogenic cell suspension culture of Picea glauca. A combination of 0.5% Cellulase R-10, 0.25% Macerozyme, 0.25% Driselase, 0.25% Rhozyme HP-150 with 0.5M mannitol and 5 mM CaCl2.2H2O produced an average of 4.5 × 10(6) protoplasts per gram fresh weight of cells. Of the several protoplast culture media tested, von Arnold and Eriksson and Kao and Michayluk (KM8P) media best supported mitotic divisions of protoplasts. A density of 10(5) protoplasts per ml and the addition of 5 mM glutamine to the culture medium was necessary to induce sustained divisions and microcallus formation. Microcalli grew into subculturable callus using a nurse culture technique.

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References

    1. Planta. 1975 Jan;126(2):105-10 - PubMed
    1. Planta. 1976 Jan;128(3):213-6 - PubMed
    1. Exp Cell Res. 1968 Apr;50(1):151-8 - PubMed
    1. Theor Appl Genet. 1980 May;56(3):97-9 - PubMed
    1. Plant Cell Rep. 1987 Feb;6(1):20-2 - PubMed

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