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. 1986 Jul 11;91(1):59-63.
doi: 10.1016/0022-1759(86)90102-x.

A flow cytometric procedure for the isolation of antigenically responsive human basophils

L M Pendy, S J Arra, L M Anselmino, L L Thomas

A flow cytometric procedure for the isolation of antigenically responsive human basophils

L M Pendy et al. J Immunol Methods. .

Abstract

A flow cytometric procedure is described for the isolation of human basophils. The basophils are isolated by cell sorting after passive sensitization with mouse FITC-IgE anti-DNP, and the isolated basophils exhibit characteristic histamine release in response to incubation with DNP21-human serum albumin conjugates. Using this method, 1-4 X 10(5) basophils can be isolated at greater than or equal to 98% purity from approximately 30 ml whole blood in a 2 h sorting procedure.

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