Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2013 May;17(2):169-75.
doi: 10.4103/0973-029X.119747.

Correlation between histological grading and ploidy status in potentially malignant disorders of the oral mucosa: A flow cytometric analysis

T Vijayavel  1 , N Aswath
Affiliations

Correlation between histological grading and ploidy status in potentially malignant disorders of the oral mucosa: A flow cytometric analysis

T Vijayavel et al. J Oral Maxillofac Pathol. 2013 May.

Abstract

Background: Histopathological grading of oral dysplastic lesions is the method of choice for evaluating malignant and potentially malignant disorders. Owing to inter- and intra-observer variability, determination of the DNA ploidy status of lesions may serve as an adjunct in the prediction of malignant transformation.

Aim: To correlate histopathological grading and ploidy status in potentially malignant and malignant disorders of the oral mucosa.

Settings and design: A pilot study was done with 30 patients (10 patients with oral potentially malignant disorders predominantly leukoplakia, 10 patients with oral malignant lesions and 10 patients with normal mucosa).

Materials and methods: Incisional biopsy was done after isolating the biopsy site with 1% Toluidine blue staining. Two sections of the tissue were removed and sent for histopathological and Flow-cytometric analysis respectively. Histopathological diagnosis was obtained and compared with Flow-cytometric results which were graded as diploid and aneuploid. Further, the S - phase fraction, DNA index were also calculated to evaluate the severity of malignant transformation or malignancy.

Statistical analysis: The results were analyzed using Pearson Chi-Square Test.

Results: There exists a significant correlation between histopathology and ploidy status in both potentially malignant and malignant group. (P = 0.002).

Conclusion: The data from this study has shown that DNA Ploidy analysis can be used as a valuable tool in assessing the carcinomatous progression of potentially malignant and malignant lesions.

Keywords: Deoxyribonucleic acid index; S-phase fraction; flow cytometer; histopathology; oral cancer; potentially malignant disorder.

PubMed Disclaimer

Conflict of interest statement

Conflict of Interest: None declared.

Figures

Figure 1
Figure 1
Deoxyribonucleic acid index calculation
Figure 2
Figure 2
Mean S-phase fraction value
Figure 3
Figure 3
Mean deoxyribonucleic acid value
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Bsoul SA, Huber MA, Terezhalmy GT. Squamous cell carcinoma of the oral tissues: A comprehensive review for oral healthcare providers. J Contemp Dent Pract. 2005;6:1–16. - PubMed
    1. Srinath Reddy K, Gupta PC. New Delhi: Ministry of Health and Family Welfare, Govt. of India; WHO; 2004. Report on Tobacco Control in India, Executive Summary. Annexure 6.
    1. Castagnola P, Malacarne D, Scaruffi P, Maffei M, Donadini A, Di Nallo E, et al. Chromosomal aberrations and aneuploidy in oral potentially malignant lesions: Distinctive features for tongue. BMC Cancer. 2011;11:445. - PMC - PubMed
    1. Epstein JB, Güneri P. The adjunctive role of toluidine blue in detection of oral premalignant and malignant lesions. Curr Opin Otolaryngol Head Neck Surg. 2009;17:79–87. - PubMed
    1. Mishra M, Mohanty J, Sengupta S, Tripathy S. Epidemiological and clinicopathological study of oral leukoplakia. Indian J Dermatol Venereol Leprol. 2005;71:161–5. - PubMed