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. 2013 Nov 20:10:343.
doi: 10.1186/1743-422X-10-343.

Human papillomavirus genotyping and integration in ovarian cancer Saudi patients

Othman A Al-Shabanah, Mohamed M Hafez  1 , Zeinab K Hassan, Mohamed M Sayed-Ahmed, Waleed N Abozeed, Salem S Al-Rejaie, Abdulmalik A Alsheikh
Affiliations

Human papillomavirus genotyping and integration in ovarian cancer Saudi patients

Othman A Al-Shabanah et al. Virol J. .

Abstract

Background: Human papillomavirus (HPV) is associated with different malignancies but its role in the pathogenesis of ovarian cancer is controversial. This study investigated the prevalence, genotyping and physical state of HPV in ovarian cancer Saudi patients.

Methods: Hundred formalin fixed paraffin embedded (FFPE) ovarian carcinoma tissues and their normal adjacent tissues (NAT) were included in the study. HPV was detected by nested polymerase chain reaction (PCR) using degenerated HPVL1 consensus primer pairs MY09/MY11 and GP5+/GP6 + to amplify a broad spectrum of HPV genotypes in a single reaction. The HPV positive samples were further genotyped using DNA sequencing. The physical state of the virus was identified using Amplification of Papillomavirus Oncogene Transcripts (APOT) assay in the samples positive for HPV16 and/or HPV18.

Results: High percentage of HPV (42%) was observed in ovarian carcinoma compared to 8% in the NAT. The high-risk HPV types 16, 18 and 45 were highly associated with the advanced stages of tumor, while low-risk types 6 and 11 were present in NAT. In malignant tissues, HPV-16 was the most predominant genotype followed by HPV-18 and -45. The percentage of viral integration into the host genome was significantly high (61.1%) compared to 38.9% episomal in HPV positive tumors tissues. In HPV18 genotype the percentage of viral integration was 54.5% compared to 45.5% episomal.

Conclusion: The high risk HPV genotypes in ovarian cancer may indicate its role in ovarian carcinogenesis. The HPV vaccination is highly recommended to reduce this type of cancer.

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Figures

Figure 1
Figure 1
PCR products were analyzed on a 2% agarose gel stained with ethidium bromide and visualized by UV-trans-illumination. Lane M is 50 PCR marker (Promega), Lanes 1, 5 and 8 are weak positive sample, lanes 2, 3, 4, 6 and 7 are negative samples, from lane 9-12 are positive samples and lane 13 is positive control.
Figure 2
Figure 2
Sequencing data of HPV genotypes (A) A sequence excised from an electropherogram for HPV type 18 (B) Sequence alignment of HPV type 16, 18 and 45 using Basic Local Alignment Search (BLAST).
Figure 3
Figure 3
The incidence of human papillomavirus in relation to ovarian cancer stages. *,# and $ indicate significant difference from Stage I, stage II and stage III respectively.
Figure 4
Figure 4
The incidence of human papillo mavirus type 16 in relation to ovarian cancer stages.
Figure 5
Figure 5
The incidence of human papillomavirus type 18 in relation to ovarian cancer stages. *, # and $ indicate significant difference from stage I, satge II and stage III, respectively.
See this image and copyright information in PMC

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