Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1984 Oct;162(4):299-304.
doi: 10.1007/BF00396740.

ATPase activity associated with isolated vacuoles of the crassulacean acid metabolism plant Kalanchoë daigremontiana

J A Smith  1 , E G Uribe, E Ball, U Lüttge
Affiliations

ATPase activity associated with isolated vacuoles of the crassulacean acid metabolism plant Kalanchoë daigremontiana

J A Smith et al. Planta. 1984 Oct.

Abstract

A technique is described that allows a relatively rapid and controlled isolation of vacuoles from leaves of the crassulacean acid metabolism (CAM) plant Kalanchoë daigremontiana. The method involves polybase-induced lysis of mesophyllcell protoplasts and isolation of vacuoles on a discontinuous density gradient. ATPase activity is associated with the isolated vacuoles and is not attributable to contamination by cytoplasmic constituents. It is suggested that this ATPase is responsible for the energization of malic-acid accumulation in the vacuole in CAM plants.

PubMed Disclaimer

References

    1. Biochim Biophys Acta. 1965 Nov 29;109(2):448-53 - PubMed
    1. Plant Physiol. 1979 Jul;64(1):74-8 - PubMed
    1. Eur J Biochem. 1984 Jun 1;141(2):415-20 - PubMed
    1. Arch Microbiol. 1975 Nov 7;105(3):319-27 - PubMed
    1. Planta. 1980 Nov;150(3):222-9 - PubMed