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. 1985 Apr;4(2):104-7.
doi: 10.1007/BF00269218.

Plant regeneration from protoplasts of long term haploid suspension cultures of N. plumbaginifolia

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Plant regeneration from protoplasts of long term haploid suspension cultures of N. plumbaginifolia

D G Barfield et al. Plant Cell Rep. 1985 Apr.

Abstract

A predominantly haploid cell suspension culture of N.plumbaginifolia has been established. The ploidy has remained stable for nearly four years in culture (and is similar in cells recovered after preservation in liquid nitrogen). Protoplasts isolated from these cells regenerate into plants with a high frequency.

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