Phenylalanine and tyrosine accumulating cell lines of a dihaploid potato selected by resistance to 5-methyltryptophan

Abstract

The selection and characterization of 5-methyltryptophan (5-MT) resistant cell lines is described in a dihaploid potato, clone H(2)578(007). The frequency of resistant calli was increased by treatment of a cell suspension with N-ethyl-N-nitroso-urea. Two lines, 5-MT-21 and 5-MT-26, accumulated tyrosine (160 nMol and 1546 nMol/g fresh callus), and the third, 5-MT-27, accumulated both tyrosine (841 nMol/g fresh callus) and phenylalanine (451 nMol/g fresh callus). In the wildtype tyrosine and phenylalanine content was 65 nMol and 42 nMol/g callus, respectively. The tryptophan content of line 5-MT-26 was significantly increased, from 20 nMol/g to 76 nMol/g fresh callus. The total free amino acid content of the three variant cell lines was higher than that of the wildtype. Variant cell lines 5-MT-21, -26 and -27 showed a low degree of resistance to 5-MT, when grown on a selective medium and were cross-resistant to parafluorophenylalanine and 3-fluorotyrosine, analogues of phenylalanine and tyrosine.