Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1985 May;69(3):329-33.
doi: 10.1007/BF00662455.

A rapid screening technique for the detection of repeated DNA sequences in plant tissues

J Hutchinson  1 , A Abbott, M O Dell, R B Flavell
Affiliations

A rapid screening technique for the detection of repeated DNA sequences in plant tissues

J Hutchinson et al. Theor Appl Genet. 1985 May.

Abstract

DNA sequences cloned from nuclear and mitochondrial chromosomes have been used as hybridisation probes to distinguish different plant genotypes. The probes are hybridised to squashed segments of tissue e.g. root tips. The 'squash-dot' method is rapid and suitable for screening large numbers of individual plants. One probe, specific for a rye repeated sequence family, enables rye chromosomes to be detected in wheat plants. A probe for ribosomal DNA enables plants with high or low numbers of ribosomal RNA genes to be distinguished. A maize mitochondrial DNA probe is used to distinguish plants with N, T or S cytoplasms.

PubMed Disclaimer

References

    1. Theor Appl Genet. 1983 May;65(2):129-44 - PubMed
    1. Nucleic Acids Res. 1980 May 10;8(9):1999-2008 - PubMed
    1. Nucleic Acids Res. 1979 Dec 11;7(7):1869-85 - PubMed
    1. Proc Natl Acad Sci U S A. 1977 Jul;74(7):2904-8 - PubMed
    1. Proc Natl Acad Sci U S A. 1975 Mar;72(3):1184-8 - PubMed