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. 1985 Aug;4(4):202-5.
doi: 10.1007/BF00269289.

Transformation of protoplast-derived cell colonies and suspension cultures by Agrobacterium tumefaciens

K Pollock  1 , D G Barfield, S J Robinson, R Shields
Affiliations

Transformation of protoplast-derived cell colonies and suspension cultures by Agrobacterium tumefaciens

K Pollock et al. Plant Cell Rep. 1985 Aug.

Abstract

In this paper we describe procedures for transforming micro colonies derived from mesophyll protoplasts of Petunia hybrida with Agrobacterium tumefaciens. The method is efficient, up to 70% of the colonies were transformed, and we used a similar method to transform cells from a suspension culture of haploid Nicotiana plumbaginifolia.

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References

    1. Plant Mol Biol. 1984 Nov;3(6):371-8 - PubMed
    1. Nature. 1965 Apr 17;206(981):302-4 - PubMed
    1. Planta. 1980 Jun;149(1):7-18 - PubMed
    1. Proc Natl Acad Sci U S A. 1983 Aug;80(15):4803-7 - PubMed
    1. Growth. 1952 Jun;16(2):65-74 - PubMed