Assignment of determinants recognized by human alloantisera to HLA-D-subregion molecules by a lysostrip technique

Abstract

We have used a lysostripping technique to investigate the molecular relationships of the determinants recognized by human alloantisera and murine monoclonal antibodies reactive with HLA-D region products. Cytotoxicity mediated by sera that define the specificities DR1, DR2, DR3, DR4, DR7, DRw11, DRw12, DRw13, DRw14, DRw52 and DRw53 was inhibited by anti-DR but not by anti-DQ Mabs. In contrast, sera that define DQw1, DQw2 and DQw3 were inhibited by anti-DQ but not anti-DR monoclonal antibodies. We found some sera used to define DR specificities that were not inhibited by monoclonal anti-DR. Cytotoxicity mediated by monoclonal antibodies reactive with the recently described specificities TA10 and MC1 was inhibited by anti-DQ and anti-DR monoclonal antibodies, respectively. The lysostripping technique is useful for the assignment of specific determinants to HLA-D subregion molecules.