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. 1982 Oct;1(5):209-11.
doi: 10.1007/BF00270237.

Somatic embryogenesis in quite a direct way in cultures of mesophyll protoplasts of Brassica napus L

L C Li  1 , H W Kohlenbach
Affiliations

Somatic embryogenesis in quite a direct way in cultures of mesophyll protoplasts of Brassica napus L

L C Li et al. Plant Cell Rep. 1982 Oct.

Abstract

Somatic embryos and plantlets have been obtained in quite a direct way from mesophyll protoplasts isolated from androgenetic plants of two cultivars ("Loras" and "Tower") of Brassica napus. The procedure consists of four steps. Proembryos were induced in a medium supplemented with a cytokinin and an auxin at comparatively high concentrations. They developed to mature embryos when the auxin was reduced or omitted.

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References

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