Infection of cowpea mesophyll protoplasts with cowpea chlorotic mottle virus (CCMV) RNA encapsulated in large liposomes
- PMID: 24257855
- DOI: 10.1007/BF00269234
Infection of cowpea mesophyll protoplasts with cowpea chlorotic mottle virus (CCMV) RNA encapsulated in large liposomes
Abstract
It has been demonstrated that cowpea chlorotic mottle virus RNA encapsulated in phosphatidyl serine/cholesterol reverse evaporation vesicles (REV) could infect cowpea mesophyll protoplasts under conditions known to enhance liposome-protoplast interactions. Positively charged phosphatidylcholine/stearylamine multilamellar liposomes did not deliver functional CCMV RNA despite their very high nucleic acid trapping capacity and their high affinity for protoplasts.
Similar articles
-
Binding of cowpea chlorotic mottle virus to cowpea protoplasts and relation of binding to virus entry and infection.Virology. 1988 May;164(1):91-8. doi: 10.1016/0042-6822(88)90623-x. Virology. 1988. PMID: 3363871
-
Double infection of tobacco protoplasts with brome mosaic virus and cowpea chlorotic mottle virus.Virology. 1980 Sep;105(2):501-7. doi: 10.1016/0042-6822(80)90050-1. Virology. 1980. PMID: 18631680
-
Small-Scale Isolation of Viral RNA-Dependent RNA Polymerase from Protoplasts Inoculated with In Vitro Transcripts.Phytopathology. 2001 Aug;91(8):747-52. doi: 10.1094/PHYTO.2001.91.8.747. Phytopathology. 2001. PMID: 18944031
-
Infectious in vitro transcripts from cowpea chlorotic mottle virus cDNA clones and exchange of individual RNA components with brome mosaic virus.J Virol. 1988 Oct;62(10):3581-8. doi: 10.1128/JVI.62.10.3581-3588.1988. J Virol. 1988. PMID: 3418781 Free PMC article.
-
Inoculation of protoplasts with viruses by electroporation.Virology. 1987 Mar;157(1):40-6. doi: 10.1016/0042-6822(87)90311-4. Virology. 1987. PMID: 18644555
Cited by
-
Infection of evacuolated turnip protoplasts with liposome-packaged cauliflower mosaic virus.Plant Cell Rep. 1985 Apr;4(2):58-62. doi: 10.1007/BF00269206. Plant Cell Rep. 1985. PMID: 24253684