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. 1983 Feb;2(1):47-50.
doi: 10.1007/BF00269235.

In vitro differentiation in callus cultures of moth bean, Vigna aconitifolia (JACQ) marechal

S Bhargava  1 , N Chandra
Affiliations

In vitro differentiation in callus cultures of moth bean, Vigna aconitifolia (JACQ) marechal

S Bhargava et al. Plant Cell Rep. 1983 Feb.

Abstract

Callus cultures of two cultivars of Vigna aconitifolia (IPCMO-926, RDM-120) were raised and their growth and differentiation studied. In IPCMO-926 callus cultures, numerous shoot buds differentiated on MS medium with BA (0.4-22.2 μM) alone or in combination with IAA (5.7 μM). In RDM-120 best differentiation of shoot buds was observed on a medium with K (23.2 μM) and IAA (5.7 μM). Kinetin alone, however, induced rhizogenesis in callus cultures. In suspension cultures of IPCMO-926 embryoids differentiated on MS medium with K (0.5 μM) and 2,4-D (0.4 and 0.9 μM).

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