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. 1983 Jun;2(3):119-21.
doi: 10.1007/BF00269333.

Mesophyll protoplasts of fenugreek (Trigonella foenumgraecum): Isolation, culture and shoot regeneration

N S Shekhawat  1 , A W Galston
Affiliations

Mesophyll protoplasts of fenugreek (Trigonella foenumgraecum): Isolation, culture and shoot regeneration

N S Shekhawat et al. Plant Cell Rep. 1983 Jun.

Abstract

High yields of mesophyll protoplasts were obtained after treatment of leaves of Trigonella foenumgraecum, a forage legume, with purified cellulase. Under appropriate conditions of culture up to 70% plating efficiency could be obtained. The protoplast-derived colonies developed into rapidly growing green calli and produced leafy shoots on a medium containing 0.1 mg/l each of benzylaminopurine (BAP) and zeatin. Addition of glutamine and asparagine to the medium was found essential for rapid cell division, callus growth and differentiation.

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References

    1. Plant Cell Rep. 1983 Feb;2(1):7-10 - PubMed
    1. Planta. 1980 Jan;147(4):340-4 - PubMed
    1. Planta. 1975 Jan;126(2):105-10 - PubMed
    1. Planta. 1970 Dec;92(4):301-8 - PubMed