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. 1983 Jun;2(3):156-9.
doi: 10.1007/BF00269343.

Aphidicolin as synchronizing agent in root tip meristems of Haplopappus gracilis

M G Galli  1 , F Sala
Affiliations

Aphidicolin as synchronizing agent in root tip meristems of Haplopappus gracilis

M G Galli et al. Plant Cell Rep. 1983 Jun.

Abstract

Aphidicolin, a reversible inhibitor of nuclear DNA replication, was tested as syncrhonizing agent in root tip meristems of Haplopappus gracilis. Embryos (i.e. decoated seeds) or 3-day-old seedlings were used to this purpose. After a 24 h treatment with the drug, a high level of synchrony was observed in both experimental materials for two cell cycles as assessed by determining the accumulation of cells in the S and M phases of the cycle. Highest synchronization was obtained with germinating embryos, possibly owing to a low degree of synchrony already existing in this system.

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