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. 1983 Aug;2(4):165-8.
doi: 10.1007/BF00270093.

A new procedure for increasing efficiency of protoplast plating and clone selection

T L Adams  1 , J A Townsend
Affiliations

A new procedure for increasing efficiency of protoplast plating and clone selection

T L Adams et al. Plant Cell Rep. 1983 Aug.

Abstract

Sea Prep(TM) agarose (FMC corp.) was used as a gelling agent for media in the culture of potato and tomato protoplasts. The concentration and conditions appropriate for gelling and liquifaction were determined. The new method provided increased calli transfer efficiency of up to 50-fold. Shoot formation from the protoplast-derived cells occurred normally and without reduction in frequency.

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References

    1. Plant Physiol. 1975 Apr;55(4):689-94 - PubMed
    1. Planta. 1975 Jan;126(2):105-10 - PubMed
    1. Planta. 1971 Mar;99(1):12-20 - PubMed

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