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. 1983 Aug;2(4):216-8.
doi: 10.1007/BF00270108.

A new method for the purification of the different stages of carrot embryoids

G Giuliano  1 , D Rosellini, M Terzi
Affiliations

A new method for the purification of the different stages of carrot embryoids

G Giuliano et al. Plant Cell Rep. 1983 Aug.

Abstract

An easy method is presented for the purification of the different stages of carrot embryoids. This is based on a synchronization of the regenerating culture and on a filtration through filters of various pore sizes. A differential sedimentation was used for removing undifferentiated cells. At the end of the process, the different stages: globular, heart- and torpedo-shaped were obtained with a degree of purity that always exceeded 90%. This method can be used for the separation of relatively large numbers of embryoids (from thousands to a million) of haploid and diploid carrot lines and is very gentle on embryoids in that it does not affect their viability or further development.

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