Microsporogenesis, reproductive behavior, and fertility in five Pennisetum species

Abstract

Microsporogenesis, reproductive behavior, pollen fertility and seed set were studied in Pennisetum basedowii Summerhayes and C. E. Hubbard, 2n = 54; P. macrostachyum (Brough.) Trin., 2n = 54; P. macrourum Trin., 2n = 36; P. polystachion (L.) Schult, 2n = 54; and P. squamulatum Fresen 2n = 54. Meiosis was regular in P. basedowii with primarily bivalent pairing. As many as 54 univalents were observed at metaphase I in P. macrostachyum. A high frequency of univalents at metaphase I in P. macrourum resulted in lagging chromosomes and micronuclei at anaphase I and telophase I, respectively. Pennisetum polystachion and P. squamulatum showed frequent multivalent chromosome associations. Studies of megasporogenesis and embryo sac development in P. basedowii showed sexual reproduction. Pennisetum macrostachyum was highly male sterile with predominantly aposporous apomictic embryo sac development. Pennisetum macrourum, P. polystachion, and P. squamulatum had only aposporous embryo sac development. Seed propagated progenies of these latter three species were uniform and matromorphic, confirming the obligate apomixis nature.