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. 1981 Dec;1(2):77-9.
doi: 10.1007/BF00269279.

Hoechst 33258 as a vital stain for plant cell protoplasts

M G Meadows  1 , I Potrykus
Affiliations

Hoechst 33258 as a vital stain for plant cell protoplasts

M G Meadows et al. Plant Cell Rep. 1981 Dec.

Abstract

A method for staining nuclei in unfixed protoplasts using Hoechst 33258 is described. It was found that the dye was not taken up by all protoplasts except in the presence of a detergent and that the staining of the nuclei was pH- and concentration-dependent. The use of vital fluorescent probes with plant protoplasts may be helpful for the identification, selection and subsequent culture of hybrid fusion products in automatic cell sorting systems.

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