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. 1985 Feb;7(1):49-54.
doi: 10.1007/BF02916546.

The effect of pH and lysosomal extract on rat liver metallothionein (Cd-thionein)

H Meulenaar  1 , C J van den Hamer, T S van den Ingh, T M Stolk
Affiliations

The effect of pH and lysosomal extract on rat liver metallothionein (Cd-thionein)

H Meulenaar et al. Biol Trace Elem Res. 1985 Feb.

Abstract

Was studied in vitro. The(109)Cd-labeled protein was isolated by gel filtration and incubated with a lysosomal extract from the same source. No degradation was seen when the pH of the incubation medium was 5 or higher, whereas the degradation of Cd-thionein was completed in 2 h at a pH of 4.5. Dissociation of Cd(2+) from thionein, which takes place to a fair extent at a pH below 5, appears necessary before the enzymatic breakdown of the thionein moiety can take place.

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