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. 1982 Jun;1(4):157-60.
doi: 10.1007/BF00269187.

Isolation of an auxotrophic cell line of soybean (Glycine max) which requires asparagine or glutamine for growth

E J Roth  1 , K G Lark
Affiliations

Isolation of an auxotrophic cell line of soybean (Glycine max) which requires asparagine or glutamine for growth

E J Roth et al. Plant Cell Rep. 1982 Jun.

Abstract

A suspension culture of haploid soybean cells was treated with ultra-violet light. From the mutagenized culture an auxotrophic cell line was isolated which grows on 1-B5 media supplemented with asparagine or glutamine. In the absence of these additives the cells cease to grow and die. Asparagine is unable to serve as a sole source of nitrogen, i.e., cannot substitute for nitrate and ammonia.

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References

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