Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2013 Dec;6(6):1744-1748.
doi: 10.3892/ol.2013.1595. Epub 2013 Sep 27.

Cloning and expression analysis of the gastric carcinoma-related gene, ELCOX3

Runliang Gan  1 , Xiaomin Liu, Yadong Zhou, Yeru Tan, Hongguang Liu, Guoqing Li, Yunlian Tang, Hailong Xie
Affiliations

Cloning and expression analysis of the gastric carcinoma-related gene, ELCOX3

Runliang Gan et al. Oncol Lett. 2013 Dec.

Abstract

Gastric cancer is a pathological process of an accumulation of multigene and multistage mutations. A new gene segment, MDSCBC11, has been previously obtained using a gene chip and is negatively associated with gastric cancer. The present study aimed to clone the full cDNA sequence of the MDSCBC11 segment and to detect its expression in gastric carcinomas and normal gastric mucosa. Multiple-tissue northern blots revealed that the new MDSCBC11-represented gene was expressed as two transcripts that were 0.8 kb and 1.5 kb in size. The cDNA sequence of the smaller transcript was 822 bp, created by 5' rapid amplification of cDNA ends (RACE) and 3' RACE methods. A bioinformatics analysis indicated that the deduced amino acid sequence of MDSCBC11 had a 99% homology with the cytochrome c oxidase III (COX3) gene in the mitochondria. A total of 46 cases of gastric carcinomas, adjacent gastric mucosa and normal gastric mucosa were individually collected, and the mRNA expression of the ELCOX3 gene was detected by RT-PCR. ELCOX3 mRNA was expressed in all 46 cases of the normal gastric mucosa. The expression levels of ELCOX3 mRNA in the gastric carcinomas were lower compared with that of the adjacent and normal gastric mucosa (P<0.05), with the percent of downregulation at 23.91% (11/46 cases). The downregulation of ELCOX3 gene expression was associated with the development of human gastric carcinomas.

Keywords: ELCOX3; MDSCBC11; cytochrome c oxidase; gastric carcinoma; gene.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Northern blot analysis of MDSCBC11 in four human fetal tissues. Lane 1, brain; 2, lung; 3, kidney; and 4, liver. The bands in the kidney and liver were ~0.8 kb and 1.5 kb in size, respectively.
Figure 2
Figure 2
DNA detection by agarose gel electrophoresis. M, DL5000; lane 1, 3′-RACE; lanes 2 and 4, 5′-RACE; lane 3, 3′-RACE control. RACE, rapid amplification of cDNA ends.
Figure 3
Figure 3
Full-length cDNA of the small transcript sequence.
Figure 4
Figure 4
Electrophoresis results of ELCOX3 mRNA expression in gastric cancer. M, DSTM2000; N1–4, normal gastric mucosa; P1–4, adjacent gastric mucosa; C1–4, gastric carcinomas. The ELCOX3 gene expression levels were low in cases 2, 3 (poorly-differentiated adenocarcinoma) and 4 (well-differentiated adenocarcinoma). There was no significant difference between the gastric carcinomas, normal gastric mucosa and adjacent gastric mucosa for case 1 (signet ring cell carcinoma).
See this image and copyright information in PMC

References

    1. Jaiswal BS, Kljavin NM, Stawiski EW, Chan E, Parikh C, et al. Oncogenic ERBB3 mutations in human cancers. Cancer Cell. 2013;13:603–617. - PubMed
    1. Otani K, Li X, Arakawa T, Chan FK, Yu J. Epigenetic-mediated tumor suppressor genes as diagnostic or prognostic biomarkers in gastric cancer. Expert Rev Mol Diagn. 2013;13:445–455. - PubMed
    1. Yoon K, Lee S, Han TS, Moon SY, Yun SM, et al. Comprehensive genome- and transcriptome-wide analyses of mutations associated with microsatellite instability in Korean gastric cancers. Genome Res. 2013;23:1109–1117. - PMC - PubMed
    1. Wang Q, Diskin S, Rappaport E, Attiyeh E, Mosse Y, Shue D, Seiser E, Jagannathan J, Shusterman S, Bansal M, Khazi D, Winter C, Okawa E, Grant G, Cnaan A, Zhao H, Cheung NK, Gerald W, London W, Matthay KK, Brodeur GM, Maris JM. Integrative genomics identifies distinct molecular classes of neuroblastoma and shows that multiple genes are targeted by regional alterations in DNA copy number. Cancer Res. 2006;66:6050–6062. - PubMed
    1. Janoueix-Lerosey I, Novikov E, Monteiro M, Gruel N, Schleiermacher G, Loriod B, Schleiermacher G, Loriod B, Nguyen C, Delattre O. Gene expression profiling of 1p35–36 genes in neuroblastoma. Oncogene. 2004;23:5912–5922. - PubMed

LinkOut - more resources